Aging changes of Macromolecular Synthesis in the Avian and Mammalian Eyes as Revealed by Microscopic Radioautography. doi: http://dx.doi.org/10.5016/1806-8774.2006v8p33

Authors

  • Tetsuji Nagata Shinshu University and Shinshu Institute of Alternative Medicine, Japan

DOI:

https://doi.org/10.5016/1806-8774.2006v8p33

Abstract

Nagata T. Aging Changes of Macromolecular Synthesis in the Avian and Mammalian Eyes as Revealed by Microscopic Radioautography. ARBS Annu Rev Biomed Sci 2006;8:33-67. In order to elucidate the aging changes of macromolecular synthesis such as DNA, RNA, proteins and glucides in various organ systems of experimental animals and men, we have studied various organs such as the skeletal, muscular, digestive, respiratory, urinary, reproductive, endocrine, circulatory, nervous and sensory systems after incorporations with radiolabeled macromolecular precursors by light and electron microscopic radioautography. This review deals with the results obtained from the avian and mammalian eyeballs. The experimental animals mainly used were chicken embryos during incubation for 1-7 days and strain mice at various aging groups from embryo to postnatal juvenile 1 and 3 days, 1 and 2 weeks, mature 1, 2 and 6 months up to 1 and 2 years at senescent stages. The animals were injected with such macromolecular precursors as 3H-thymidine for DNA, 3H-uridine for RNA, 3H-amino acids for proteins, 3H-glucose, 3H-glucosamine and 35S-sulfate for glucides. The results demonstrated that these precursors were incorporated into various ocular cells and tissues at various aging groups from perinatal to juvenile, mature and senescent stages showing specific patterns of macromolecular synthesis. It is concluded that the difference of macromolecular syntheses during development and aging changes were demonstrated.

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Published

2007-01-31

Issue

Vol. 8 (2006)

Section

Reviews

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